First Report of the Endophytic Bacteria Associated with Phormidium sp.

Recent molecular studies on endophytic bacterial diversity have revealed a large richness of species. Associations between endobiotic bacterial-algae interactions been studied for more than 40 years but were, up to now, never molecularly analyzed within the filamentous Cyanobacteria Phormidium. Therefore, bacteria associated with fresh microalgae Phormidium, group ubiquitous photosynthetic organisms that play an important role in aquatic ecosystems, has investigated. To study this partnership, Phormidium sp. was cultured BG-11 medium using optimal conditions, and after incubation period, cell biomass obtained. Total genomic DNA from extracted used determination by 16S rRNA gene. Sequencing results total seven living cytoplasm host identified, including six belonging three genera, namely Sphingomonas, Sphingopyxis, Stenotrophobacter while one remained unidentified due low sequence homology GenBank database. The highlighted importance first time sequence-based identification.